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Alkanes Data in Seawater and in Diluted Chemically Enhanced Water-Accommodated Fraction (WAF) of Oil for LTOPOS, a Long-Term Oil and Particle Oxidation Study (Mesocosom 7)

Authors: Liu, Yina and Terry Wade

Published On: Aug 07 2020 19:04 UTC

DOI: https://doi.org/10.7266/3EGKX98Y

UDI: R6.x807.000:0061

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Project Information

Funded By:
Gulf of Mexico Research Initiative

Funding Cycle:
RFP-VI

Research Group:
Aggregation and Degradation of Dispersants and Oil by Microbial Exopolymers 2 (ADDOMEx-2)

Point of Contact

Terry Wade
Texas A&M University / Geochemical and Environmental Research Group
terry@gerg.tamu.edu

Theme keywords

oil spill, biodegradation, microbes, marine snow, metabolites, PAHs, alkanes, gas chromatography mass spectrometry (GC/MS), Diluted Chemically Enhanced Water Accommodated Fraction, DCEWAF, pristane, phytane, microbial oxidation, crude oil

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Abstract:

Mesocosm experiments were performed with seawater collected from Galveston Bay, Texas. Alkanes were measured in filtered samples from two treatments: 1) control (seawater) and 2) diluted chemically enhanced water-accommodated fraction of crude oil. Alkanes from two fractions were collected: particle associated phase and dissolved phase. The extracted samples were analyzed using gas chromatography mass spectrometry (GC/MS).

Suggested Citation:

Liu, Yina and Terry Wade. 2020. Alkanes Data in Seawater and in Diluted Chemically Enhanced Water-Accommodated Fraction (WAF) of Oil for LTOPOS, a Long-Term Oil and Particle Oxidation Study (Mesocosom 7). Distributed by: Gulf of Mexico Research Initiative Information and Data Cooperative (GRIIDC), Harte Research Institute, Texas A&M University–Corpus Christi. doi:10.7266/3EGKX98Y

Purpose:

To determine the concentration of alkanes during microbial oxidation of crude oil components.

Data Parameters and Units:

Data Parameters: alkanes (n-C10, n-C11, n-C12, n-C14, n-C15, n-C16, n-C17, n-C18, n-C19, n-C20, n-C21, n-C22, n-C23, n-C24, n-C25, n-C26, n-C27, n-C28, n-C29, n-C30, n-C31, n-C32, n-C33, n-C34, n-C35, n-C36, Pristane, Phytane); DCEWAF (Diluted Chemically Enhanced Water Accommodated Fraction)-Units: ng/L

Methods:

Alkanes were collected for both particle associated phase and dissolved phase. Dissolved phase was collected for each sample passing through a filtration series (GF/D and then 0.2µm). The particle associated phase is defined as particles stayed on the GF/D and 0.2 µm filters. The dissolved phase for each sample was preserved in dichloromethane and extracted with liquid-liquid extraction with dichloromethane. The particle associated phase (i.e. filters) samples were stored at -20 ℃ before extraction. The filters for each sample (GF/D and 0.2 µm) were combined and extracted with dichloromethane. The extracted samples were analyzed on gas chromatography mass spectrometry. Particle associated phase concentrations were normalized to the volumes of water passed through the filters. Triplicate samples were collected for each treatment at four (4) time intervals: Day 0, 4, 8, and 16.

Instruments:

gas chromatography mass spectrometry (GC/MS)