Abstract:

Various biochemicals that are induced when fish and other organisms are exposed to petroleum hydrocarbons serve as ideal biomarkers of oil exposure. Here, bile fluorescence at 290/330 nm is presented, along with liver ethoxy resorufin-o-deethylase activity (EROD), liver superoxide dismutase activity, and liver glutathione-s-transferase activity as measurements of phase 1 and phase 2 detoxification and oxidative defenses.

Suggested Citation:

Patterson III, William F, Joseph Tarnecki, and Charles Jagoe. 2015. Reef fish biomarker data from bile and liver at natural reefs sites in the northern Gulf of Mexico from November 2010 - August 2011. Distributed by: Gulf of Mexico Research Initiative Information and Data Cooperative (GRIIDC), Harte Research Institute, Texas A&M University–Corpus Christi. doi:10.7266/N7B27S7H

Purpose:

To identify and measure biomarkers which are present as a result of oil exposure.

Data Parameters and Units:

Sample ID (unique sample identifier), Date (date sample collected MM/DD/YYYY), Species (common name) [Amberjack, Leopard Toadfish, False Albacore, Red Snapper, Vermillian Snapper, Gray Triggerfish, Scamp, Red Porgy, Tomtate, Speckled Hind, Creole Fish, Bank Seabass, Sand Perch, Red Drum, Yellowedge Grouper, Red Grouper, Black Seabass, Bigeye, Cobia, Southern Puffer, Blackfin Tuna, King Mackerel, Longtail Bass, Spinycheek Scorpionfish, Tilefish, Ocellated Flounder, Inshore Lizardfish, Tattler Bass], Site (reef name), Bile Fluorescence at 290/330 nm (relative fluorescence units, dilution corrected), Liver ethoxy resorufin-o-deethylase activity (pmol resorufin per min per mg protein), liver superoxide dismutase activity (per mg protein), and liver glutathione-s-transferase activity (nmol min mg protein).

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